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Sample Setup: Fluorescence

Overview

Fluorescence measurements require a sensitive detector and an effective filter for discriminating between powerful excitation source wavelengths and weak spectral emissions from the sample.

Spectrometer

We offer several spectrometers useful for fluorescence, but recommend the high-sensitivity, preconfigured USB4000-FL Spectrometer for most general fluorescence applications. The USB4000-FL is set to 360-1000 nm and comes with a 200-µm slit and an L4 Detector Collection Lens for increased light throughput.

Sampling Optics

Your standard excitation source option is our PX-2 Pulsed Xenon Source. Our proprietary LVF Linear Variable Filters are excellent tools for spectrally shaping the excitation energy from broadband sources used for fluorescence. Various sampling optics are available for detecting picomolar-range concentrations of fluorophores from surfaces and in solutions and powders.

Ordering Information

Spectrometer Components	Price
USB4000-FL Spectrofluorometer	$2887
200 µm Slit as entrance aperture	included
Grating #3, 380-1000 nm range	included
L4 Detector Collection Lens	included
SAG+UPG Mirrors	$290

Components for Use with Solutions	Price
PX-2 Pulsed Xenon Source	$889
CUV-ALL-UV 4-way Cuvette Holder	$935
LVF-UV-KIT Linear Variable Filter Kit	$1154
QP1000-2-UV-VIS Premium-grade Patch Cord Assemblies (price is for one; two are recommended)	$415
74-MSP Mirrored Screw Plugs (price is for one; two are recommended)	$115
SpectraSuite Spectroscopy Operating Software	$199

Components for Use with Solids	Price
PX-2 Pulsed Xenon Source	$889
R400-7-VIS-NIR Reflection/Backscattering Probe	$577
SpectraSuite Spectroscopy Operating Software	$199

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