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Sample Setup: Gas Absorbance

Overview

Absorbance measurements are used to quantify the concentration of solutions and gases (as described here) that absorb light in a media that transmits light. The signal in absorbance units is proportional to the molar absorptivity, pathlength and concentration of the sample. (See more on Beer’s Law.)

Spectrometer

A setup for measuring benzene gas, for example, would call for an HR4000 High-resolution Spectrometer with an H7 grating and a 200-300 nm wavelength range. Optical bench accessories include an L4 Detector Collection Lens for increased light throughput, and a UV4 Detector Upgrade to transmit light in the UV. With a 5 µm slit, optical resolution of ~0.07 nm (FWHM) is possible. The preferred light source for work in the ultraviolet is the D2000 Deuterium Light Source.

Sampling Optics

The 10-cm pathlength CUV-UV-10 Cuvette Holder, the CV-Q-10 Cylindrical Cell and QP400-025-SR Premium-grade Solarization-resistant Optical Fibers (one fiber illuminates, the other reads signal) comprise the system’s sampling optics. For applications requiring shorter pathlengths or open-air monitoring, use an optical fibers-and-collimating lenses configuration.

Ordering Information

Spectrometer Components	Price
HR4000 High-resolution Spectrometer	$4619
Grating H7, 2400 lines per mm, 200-300 nm range	included
5 µm Slit as entrance aperture	$174
L4 Detector Collection Lens	$174
DET4-UV Detector with UV4 Detector Upgrade	$174

Additional Sampling Components	Price
D2000 Deuterium Light Source	$2563
CUV-UV-10 Cuvette Holder	$635
CV-Q-100 Cylindrical Cell	$174
QP400-2-UV-VIS Premium-grade Patch Cord Assemblies (price is for one assembly, two are recommended)	$196
SpectraSuite Spectroscopy Operating Software	$199
ASP Annual Service Package	Call for Price

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