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Ocean Optics - Inventor of the World's First Miniature Spectrometer
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Ocean Optics Linear Variable
Filters Reshape
Traditional Fluorescence Spectroscopy Techniques
Novel LVF-HL and LVF-UV-HL Filters Replace
Expensive Filters and Spectrally Shape Broadband Excitation Energy
Dunedin, Fla.,
September 2004 – Ocean Optics patented LVF-HL and LVF-UV-HL Linear
Variable Filters are changing the way that scientists approach traditional
fluorescence spectroscopy measurements by eliminating the need for
multiple, expensive bandpass filters and by spectrally shaping the
excitation energy from a single broadband source. A single LVF cassette
($499) can replace multiple filters that often cost more than $100 each.
Additionally, LVF sets enable the user to shape the excitation energy from
a single broadband source as opposed to using multiple light sources with
various wavelengths or a front-end scanning monochromator.
The LVF-HL
(300-750 nm) and the LVF-UV-HL (230-500 nm) are high-pass and low-pass
filters fastened together in a single filter cassette that can be inserted
easily into a fluorescence setup using a variety of LVF accessories. Each
LVF cassette features an adjustable center wavelength and an adjustable
bandpass across the filter’s effective range. The filters are pre-set
with a transmission bandwidth at 25 nm, but easy-to-adjust screws allow
the user to create a transmission bandwidth as wide as 100 nm or as narrow
as 20 nm by positioning the filters against one another.
Additionally, LVF
slides can be used on the emission side of a fluorescence experiment to
better discriminate between a fluorophore’s weak emission wavelengths
and those of a more powerful excitation source.
The LVF-HL and
LVF-UV-HL feature excellent transmission bands at ~90% and blocking bands
~99.8%. The quartz filters, coated with an interference pattern, are only
57 mm x 10 mm.
In addition to
the LVF-HL and LVF-UV-HL, Ocean Optics offers a variety of patented Linear
Variable Filters including single high- and low-pass filters, and double
high- and low-pass filters that increase the blocking band to 99.96%.
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Contact
an Ocean Optics Applications Scientist
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