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SF2000 Spectrofluorometer

Conceived for use with our Fiber Optic Oxygen Sensors, the SF2000 Miniature Fiber Optic Spectrophotometer is a low-cost, high-sensitivity spectrometer configured especially for measuring fluorescence.

The SF2000 is no longer available. It has been replaced with several other fluorescence spectrometers:

At the heart of the SF2000 is an S2000 Series Fiber Optic Spectrometer pre-configured to a 360-1000 nm wavelength range (click here for grating efficiency), with a 200 µm entrance slit (for optical resolution to ~10.0 nm FWHM ) and L2 Detector Collection Lens (for increased light throughput). Unlike the S2000-FL Spectrometer for fluorescence, which is similarly configured, the SF2000 also features an integral R-LS-450 card-mounted Pulsed Blue LED. Both spectrometer and LED are mounted in an SD2000 dual-channel spectrometer housing (see photo).

The SF2000 interfaces to a desktop PC via Ocean Optics' ADC1000 ISA-bus A/D converter. Another interface option is the ADC1000-USB, an external A/D converter with 1 MHz sampling frequency that connects to the spectrometer via a DB25-pin connector and to desktop or notebook PCs via the USB port. A PCI-bus converter is also available.

The SF2000's integral R-LS-450 Pulsed Blue LED uses an external power source but has internal connections for synchronization with the spectrometer.

The SF2000 Spectrometer has a compact optical bench coupled to a high-sensitivity 2048-element linear CCD-array detector. The spectrometer accepts light energy transmitted through single-strand optical fiber and disperses it via a fixed grating (set for 360-1000 nm) across the CCD array. An SMA 905 connector allows for easy coupling to Fiber Optic Oxygen Sensors and other fiber optic accessories.

Specifications

Detector Specifications

Detector:	2048-element linear silicon CCD array
CCD elements:	2048 elements @ 14 µm x 200 µm per element
Well depth (@ 600 nm):	160,000 photons
Sensitivity (estimated):	86 photons/count 2.9 x 10-17 joule/count 2.9 x 10-17 watts/count (for 1-second integration)
Detector range:	200-1100 nm
Useable range:	360-1000 nm
Integration time:	4 milliseconds to 60 seconds (with 500 kHz A/D converter) 20 milliseconds to 60 seconds (with 100 kHz PCMCIA A/D converter)

For the SF2000, performance specifications reflect a configuration that includes a 600 lines/mm grating, 200 µm entrance slit and L2 Detector Collection Lens.

Optics Specifications

Grating:	600 lines/mm, set to 360-1000 nm (blaze wavelength is 500 nm)
Slit:	200 µm width (height is 1 mm)
Resolution:	~10.0 nm (FWHM)
Stray light:	< 0.05% at 600 nm < 0.10% at 435 nm
Fiber optic connector:	SMA 905 to single-strand optical fiber (0.22 NA)

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Convenient Wavelength Calibration!
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