Ocean Optics - Inventor of the World's First Miniature Spectrometer
Ocean Optics - Inventor of the World's First Miniature Spectrometer

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Ocean Optics - Inventor of the World's First Miniature Spectrometer


QE65000 Scientific-grade Spectrometer

Note: QE65000 has been replaced by QE65 Pro. Learn more here.

The QE65000 Scientific-grade Spectrometer is a novel combination of detector, optical bench and electronics technologies that provides users with a remarkably sensitive system for demanding low-light level applications such as fluorescence, DNA sequencing, astronomy and Raman spectroscopy.  The QE65000 can achieve up to 90% quantum efficiency with high signal-to-noise and rapid signal processing speed.

New! Optical Bench Enhancements

The great performance of our QE65000 Spectrometer has been enhanced to provide even more value: lower stray light, improved efficiency in the UV and Shortwave NIR, and better unit-to-unit reproducibility. For full UV-Shortwave NIR coverage, you'll want to configure your QE65000 with our HC-1 composite grating.

Quantum Efficiency to 90%

The QE65000 is the most sensitive spectrometer we have ever developed. The Hamamatsu FFT-CCD detector used in the QE65000 provides 90% quantum efficiency (defined as how efficiently a photon is converted to a photo-electron). Most of our other detectors are linear CCDs but with this “2D” area detector, we can bin (or sum) a vertical row of pixels, which offers significant improvement in the signal-to-noise ratio (>1000:1) and signal processing speed of the detector compared with a linear CCD, where signals are digitally added by an external circuit.

Increased System Sensitivity

In our spectrometers with linear CCDs, the slit’s width, not its height, regulates the amount of light entering the bench because linear CCDs cannot efficiently collect the light from the entire height of the slit. But in the QE65000, the 2D area detector can better take advantage of the height of the slit and the additional light, greatly improving system sensitivity.

Excellent UV Response

Because the detector in the QE65000 is back-thinned (or back-illuminated), it has great native response in the UV and does not require the additional coatings that we typically apply to other detectors for UV applications.

Demanding Low Light-level Applications

The QE65000 Spectrometer is a great option for low-light level applications including fluorescence, Raman spectroscopy, DNA sequencing, astronomy and thin-film reflectivity. The TE-cooled (down to -15 °C) detector features low noise and low dark signal, which enables low-light-level detection and long integration times (analogous to the shutter speed of a camera) from 8 milliseconds to 15 minutes, with virtually no spectral distortion.


An example of a fluorescence setup with a QE65000 uses the PX-2 Pulsed Xenon Lamp as an excitation source and the CUV-ALL Cuvette Holder for samples. An optical fiber delivers excitation light to the sample holder and read light to the spectrometer. A filter, such as one of our Linear Variable Filters would block excitation light from entering the spectrometer

Onboard Programming

The QE65000 also has an onboard programmable micro-controller for controlling the spectrometer and accessories. You have access to 10 user-programmable digital inputs/outputs and a pulse generator for triggering other devices. You can use the I/Os to flash a lamp, stop/start a process, and send a message/alarm during the spectrometer’s integration period. The spectrometer’s operating parameters can be controlled through software. In fact, wavelength calibration coefficients unique to each spectrometer are programmed into a memory chip right on the spectrometer.

Optical Bench Options

At Ocean Optics, we understand that your work is unique and that you want a spectrometer to fit your specific needs. With the QE65000 Spectrometer, you can configure the bench for your application. Choose QE65000 Optical Bench Options such as entrance aperture size, detector accessories, filters, gratings and more. Our application scientists will help you determine which configuration will best work for you.

Variety of Filter Options Available

We have a number of variable bandpass order-sorting filter options available to vary the starting wavelength at 50 nm intervals to fit your particular application. These OFLVs are for use with the HC-1 grating only and consist of the following:

OFLV Filter Wavelength Range
OFLV-QE 200-950 nm
OFLV-QE-250 250-1000 nm
OFLV-QE-300 300-1050 nm
OFLV-QE-350 350-1100 nm
OFLV-QE-400 400-1150 nm

Triggering Options

The QE65000 is available with external triggering that helps you easily synchronize data acquisition with external events. Check out the documentation (PDF format):

Installation and Operation Manual

Our QE65000 Manual provides you with instructions for setting up, calibrating and performing experiments with your QE65000 spectrometer.



Dimensions: 182 mm x 110 mm x 47 mm
Weight: 1.18 kg (without power supply)


Hamamatsu S7031-1006

Detector range: 200-1100 nm


1024 x 58 (1044 x 64 total pixels)

Pixel size:

24 μm2

Pixel well depth:

1000 Ke-

Sensitivity: 22 electroncs/count all ٨; 26 photons/count at 250 nm
Quantum efficiency: 90% peak; 65% at 250 nm
Optical Bench
Design: f/4, Symmetrical crossed Czerny-Turner
Focal length: 101.6 mm input and output
Entrance aperture: 5, 10, 25, 50, 100 or 200 µm wide slits or fiber (no slit)
Grating options: Multiple grating options, UV through Shortwave NIR
HC1-QE grating option: provides UV-Shortwave NIR coverage
Detector collection lens option: None
OFLV filter options: OFLV-QE (200-950 nm); OFLV-QE-250 (250-1000 nm); OFLV-QE-300 (300-1050 nm); OFLV-QE-350 (350-1100 nm); OFLV-QE-400 (400-1150 nm)
Other bench filter options: Longpass OF-1 filters
Collimating and focusing mirrors: Standard only
UV enhanced window: No
Fiber optic connector: SMA 905 to 0.22 numerical aperture single-strand optical fiber
Wavelength range: Grating dependent
Optical resolution: ~0.14-7.7 nm FWHM
Signal-to-noise ratio: 1000:1 (at full signal)
A/D resolution: 16 bit
Dark noise: 3 RMS counts
Dynamic range: 7.5 x 109 (system), 25000:1 for a single acquisition
Integration time: 8 ms to 15 minutes
Stray light: <0.08% at 600 nm; 0.4% at 435 nm
Corrected linearity: >99%
Power consumption: 500 mA @ 5 VDC (no TE cooling); 3.5 A @ 5 VDC (with TE cooling)
Data transfer speed: Full scans to memory every 8 ms with USB 2.0 port, 18 ms with USB1.1 port, 300 ms with serial port
Inputs/Outputs: 10 onboard digital user-programmable GPIOs (general purpose inputs/outputs)
Analog channels: No
Auto nulling: Yes
Breakout box compatibility: Yes, HR4-BREAKOUT
Trigger modes: 4 modes
Strobe functions: No
Gated delay feature: Yes
Connector: 30-pin connector
Power-up time: <5 seconds

Dark current:

4000 e-/pixel/sec @ 25 ºC; 200 e-/pixel/sec @ 0 ºC

Operating systems: Windows 98/Me/2000/XP, Mac OS X and Linux with USB port; Any 32-bit Windows OS with serial port
Computer interfaces: USB 2.0 @ 480 Mbps; RS-232 (2-wire) @ 115.2 K baud
Peripheral interfaces: SPI (3-wire); I2C inter-integrated circuit
Temperature and Thermoelectric (TE) Cooling
Temperature limits: 0 ºC to 50.0 ºC; no condensation
Set point: Software controlled; lowest set point is 40 ºC below ambient
Stability +/-0.1 ºC of set temperature in <2 minutes

Systems starting at $11,000. Ask an Ocean Optics Applications Scientist about configuration options, software requirements and optional accessories.

Exclusive 3-Year Warranty

Like all Ocean Optics spectrometers and systems*, the QE65000 Series is covered under our 3-Year Warranty against manufacturing defects. No other manufacturer offers a stronger guarantee. Read more here.

* OEM equipment excluded

Contact an Ocean Optics Applications Scientist

Predicted Ranges and Resolutions



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